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. Author manuscript; available in PMC: 2018 Jan 19.
Published in final edited form as: Leukemia. 2013 Sep 11;28(1):138–146. doi: 10.1038/leu.2013.260

Fig. 2. Chromatin characteristics of MEIS1 regulatory elements in mammalian hematopoietic cells.

Fig. 2

A). HSPC cells (Lin- CD34+ c-Kit+) were isolated from mouse bone marrow by FACS.

B). mRNA expression of c-Kit, Gr1, and Meis1 were detected in HSPC population.

C). H3K27ac enhancer marks of the Meis1 gene during hematopoiesis in mice. ChIP assay was performed with the antibody against H3K27ac in HSPC cells. The amounts of input DNA and ChIP DNA were normalized, and the data are shown as the relative enrichment ratio of precipitated DNA to input DNA. P1 is in the promoter of Meis1 gene, and D130 is located 140kb down stream of the 3′ of Meis1 gene.