Table 1. Effect of time on 280 nm absorbance of native IgG and its modified counterparts.
| Sample | Absorbance at 280 nm | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| (0 h) | (24 h) | (48 h) | (72 h) | (96 h) | (120 h) | (144 h) | (168 h) | (192 h) | |
| Native IgG | 0.80 (0.0) | 0.82 (0.0) | 0.84 (0.0) | 0.85 (0.0) | 0.86 (0.0) | 0.87 (0.0) | 0.88 (0.0) | 0.88 (0.0) | 0.88 (0.0) |
| IgG + 5 mMglucose | 1.4 (42.85) | 1.46 (43.83) | 1.54 (45.45) | 1.61 (47.20) | 1.72 (50.00) | 1.8 (51.66) | 1.84 (52.17) | 1.91 (53.92) | 1.86 (52.68) |
| IgG + 10 mM glucose | 1.5 (46.66) |
1.55 (47.09) | 1.62 (48.14) | 1.7 (50.00) | 1.8 (52.22) | 1.9 (54.21) |
1.96 (55.10) | 2.01 (56.21) | 1.9 (55.55) |
| IgG + 5 mM glucose +3.33 μM MGO | 1.54 (48.05) | 1.62 (49.38) | 1.7 (50.58) | 1.78 (52.24) | 1.86 (53.76) | 1.95 (55.38) | 2.03 (56.65) | 2.1 (58.09) | 2.05 (57.07) |
| IgG + 5 mM glucose +6.67 μM MGO | 1.64 (51.21) | 1.74 (52.87) |
1.81 (53.59) | 1.91 (55.49) | 1.99 (56.78) | 2.06 (57.76) | 2.13 (58.68) | 2.2 (60.00) | 2.16 (59.25) |
| IgG + 10 mM glucose +3.33 μM MGO | 1.72 (53.48) | 1.82 (54.94) | 1.89 (55.55) | 2.02 (57.92) | 2.15 (60.00) | 2.27 (61.67) |
2.39 (63.17) |
2.54 (65.35) | 2.49 (64.65) |
| IgG + 10 mM glucose +6.67 μM MGO | 1.82 (56.04) | 1.94 (57.73) | 2.1 (60.00) | 2.17 (60.82) | 2.29 (62.44) | 2.49 (65.06) | 2.64 (66.66) | 2.75 (68.00) | 2.67 (67.04) |
Note: The data within parentheses indicate percent change in absorbance at 280 nm compared to native IgG.