Table 2. Effect of time on tryptophan specific fluorescence of various samples.
| Sample | Fluorescence intensity (F.I.) | |||||||
|---|---|---|---|---|---|---|---|---|
| (0 h) | (24 h) | (48 h) | (72 h) | (96 h) | (120 h) | (144 h) | (168 h) | |
| Native IgG | 980 | 950 | 917 | 880 | 848 | 800 | 776 | 760 |
| IgG + 5 mM glucose | 934 (4.69) | 900 (5.26) | 866 (5.56) | 824 (6.36) | 792 (6.60) |
745 (6.87) | 721 (7.08) |
700 (7.89) |
| IgG + 10 mM glucose | 912 (7.45) |
872 (8.21) | 816 (11.01) | 743 (15.56) | 706 (16.74) | 657 (17.87) | 540 (30.41) | 490 (35.52) |
| IgG + 5 mM glucose + 6.67 μM MGO |
700 (28.57) | 646 (32.00) | 606 (33.91) | 576 (34.54) | 516 (39.15) | 442(44.75) | 396 (48.96) | 320 (57.89) |
| IgG + 10 mM glucose + 6.67 μM MGO | 600 (38.77) | 564 (40.63) | 514 (43.94) | 478 (45.68) | 422 (50.23) |
376 (53.00) | 300 (61.34) | 260 (65.78) |
Note: The data within parentheses indicate percent decrease in F.I. compared to native IgG.