Table 3. Effect of time on AGEs specific fluorescence of various samples.
| Sample | Fluorescence intensity (F.I.) | |||||||
|---|---|---|---|---|---|---|---|---|
| (0 h) | (24 h) | (48 h) | (72 h) | (96 h) | (120 h) | (144 h) | (168 h) | |
| Native IgG | 55 | 65 | 75 | 85 | 85 | 85 | 85 | 85 |
| IgG+ 5 mM glucose | 100 (45.00) | 130 (50.00) | 250 (70.00) | 320(73.43) | 380 (77.63) | 440 (80.68) | 480 (82.29) | 510 (83.33) |
| IgG+ 10 mM glucose | 125 (56.00) | 200 (67.50) | 340 (77.94) | 420 (79.76) | 480 (82.29) | 575 (85.21) | 640 (86.71) | 700 (87.85) |
| IgG + 5 mM glucose + 6.67 μM MGO | 250 (78.00) | 350 (81.42) | 440 (82.95) | 530 (83.96) | 615 (86.17) | 680 (87.50) | 740 (88.51) | 800 (89.37) |
| IgG + 10 mM glucose + 6.67 μM MGO | 325 (83.07) | 450 (85.55) | 595 (87.39) | 690 (87.68) | 720 (88.19) |
760 (88.81) | 810 (89.50) | 860 (90.11) |
Note: (i) The data within parentheses indicate percent increase in F.I. compared to native IgG. (ii) All samples were excited at AGEs specific wavelength of 370 nm.