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. 2018 Jan 19;13(1):e0191318. doi: 10.1371/journal.pone.0191318

Fig 2. Characteristics of tail-suspended mice and aged mice.

Fig 2

(A) Twelve-week-old C57BL/6J male mice were subjected to two weeks of tail suspension. Mice were sacrificed, and then serum samples and soleus muscles were collected. Serum IL-6 level was determined using ELISA. Soleus muscle weight/body weight ratio and the body weight of mice are shown (n = 4). (B) Twelve-week-old mice were subjected to seven days of tail suspension, and then RNA was extracted from the following tissues: whole bone marrow from femurs (n = 4), soleus muscles (n = 3), livers (n = 6), spleens (n = 3), and small intestines (n = 3). Quantitative real-time PCR was performed, and the relative IL-6 expression in each tissue is shown. (C) C57BL/6J male mice aged 15 weeks and 104 weeks were sacrificed, and then serum samples and soleus muscles were collected (n = 4). *p<0.05, **p<0.01, n.s.: not significant.