Table 1. Primer and PCR conditions used for amplification and sequencing of VGCS gene of Aedes albopictus (Kasai et al., 2011).

Domains	Primer name	PCR Primers (5'-3')	PCR condition	Sequencing primers (5'-3')
II	aegSCF20	GACAATGTGGATCGCTTCCC	Initial denaturation at 94°C for 3 min, 35 cycles each of 94°C for 15 s, 55°C for 30 s, and 72°C for 30 s, followed by a final elongation step at 72°C for 10 min	aegSCF3: GTGGAACTTCACCGACTTCA
	aegSCR21	GCAATCTGGCTTGTTAACTTG		aegSCR22: TTCACGAACTTGAGCGCGTTG
III	aegSCF7	GAGAACTCGCCGATGAACTT		aegSCR8: TAGCTTTCAGCGGCTTCTTC
	aegSCR7	GACGACGAAATCGAACAGGT
IV	albSCF6	TCGAGAAGTACTTCGTGTCG		albSCF7: AGGTATCCGAACGTTGCTGT
	albSCR8	AACAGCAGGATCATGCTCTG