Table 1. Antigenic integrity of EDIIIs on DSV4 VLPs using sandwich ELISA.

S.No.a	Anti-EDIII mAbs b	Strongly Neutralizes	Region specificity	ELISA OD	S/Co Ratioc	Reactivityd
1	E103	DENV-1	BC loop (LR)	3.96	14.4	++++
2	3H5	DENV-2	A strand, BC loop (LR)	0.77	2.8	+
3	70		A strand, LR	0.48	1.75	+
4	106		A strand, LR	0.51	1.85	+
5	104		C strand/C-C’ loop	0.52	1.89	+
6	8A1	DENV-3	N terminus of A strand, FG loop (LR)	1.30	4.72	++
7	E51		LR	3.90	14.18	++++
8	E88	DENV-4	BC, DE loops (LR)	0.53	1.92	+
9	E76	DENV-2, -4	N terminus of A strand, C-C’ loop, B, D & G strands	0.51	1.85	+
10	E106	DENV-1, -4	A strand, LR	3.60	13.09	++++
11	E113	DENV-1, -2, -4	LR	1.20	4.36	++
12	h-2J20	DENV-1, -3	?	2.50	9.09	+++
13	E61	DENV-1, -2, -3, -4	A and G strands	0.71	2.58	+
14	E77		A strand, BC loop (LR), and G strand	0.81	2.95	+
15	h-2K2	DENV-1, -2, -3, -4	prM	0.06	0.22	-
16	4G2		FL	0.05	0.18	-
17	h-1M7			0.06	0.22	-
18	h-DVC23.3			0.05	0.18	-
19	3H4			0.05	0.18	-
20	h-1N5			0.05	0.18	-

^aS.Nos. 1–14: Anti-EDIII mAbs; S.Nos. 15–20: Non-EDIII mAbs

^bmAbs are described in the following references: h-DVC23.3 [16]; h-2K2 [21]; E103, E106 & E113 [29]; 3H5 & 4G2 [49]; 8A1 [50]; E51, E61, E77 [51]; 3H4 & h-2J20 [52]; h-1M7 & h-1N5 [53]; 70, 104 & 106 [54]; E76 & E88 [55].

^cS/Co: Signal (ELISA OD)/Cut-off; Cut-off = 0.275 (mean ELISA OD obtained using S antigen with each of the 20 mAbs plus 3x SD).

^dReactivity was graded based on values of S/Co:—(<1); + (1.1–3.0); ++ (3.1–5.0); +++(5.1–10); ++++ (>10.1)

?: unknown