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. 2017 Nov 8;159(2):609–621. doi: 10.1210/en.2017-00695

Figure 3.

Figure 3.

TF, but not TBT, inhibits pyruvate respiration in an MPC2-dependent manner. (A) Mpc2 floxed mice were crossed with the adiponectin promoter–driven Cre-expressing mouse to create FS-Mpc2−/− mice. (B) Western blotting confirmed MPC2−/− in BAT from FS-Mpc2−/− mice. (C) The effects of TBT (5 µM) on BAT mitochondrial pyruvate and succinate use from WT and FS-Mpc2−/− mice. Sequential addition of pyruvate/ADP (black), TBT (gray), and succinate (white). Upon addition of TBT, both WT and FS-Mpc2−/− mitochondria decreased oxygen consumption rate (OCR) compared with their perspective pyruvate/ADP addition. *Indicates P < 0.05 versus WT vehicle. (D) The effects of TF (5 µM) on mitochondrial pyruvate and succinate use from WT and FS-Mpc2−/− mice. Sequential addition of pyruvate/ADP (black), TBT (gray), and succinate (white). Upon addition of TF, FS-Mpc2−/− mitochondria did not alter OCR; however, WT mitochondria decreased OCR compared with its pyruvate/ADP addition. *Indicates P < 0.05 versus WT vehicle. (E) The effects of DEX (5 µM) on mitochondrial pyruvate–stimulated respiration. Data are presented as OCR [pmol/(s⋅mg)]. Ethanol was used as the vehicle control. (F) The effects of TF (5 µM) on mitochondrial pyruvate–mediated respiration in mitochondria isolated from a variety of tissues. *Indicates P < 0.05 versus vehicle. Data are presented as OCR [pmol/(s⋅mg)]. Ethanol was used as the vehicle control. KO, knockout.