Figure 1.

Reciprocal regulation of ephrin-A1 and EphA2 expression in human and mouse cornea. Frozen corneal tissue sections from human cadavers (A) and wild-type Balb/C mice (B) were immunostained with antibodies against EphA2 or ephrin-A1 (red, bottom). DAPI (blue) was used to highlight nuclei. (A) Arrowheads indicate the limbus–cornea junction where the limbus ends and the cornea begins. (B) Mouse eyelids are marked as a reference point for limbal tissue orientation. Arrows show concentrated ephrin-A1 staining and paucity of EphA2 staining in the limbus. White dotted lines demarcate the basement membrane region. CC, central cornea; L, limbus. n = 3. Scale bar denotes 100 μm.