TABLE 2.
Detection of HCV RNA positive and negative strands in CD4+ and CD8+ T cells exposed to authentic, patient-derived HCVc
| Expt | HCV inoculum | T cell subtype | No. of target cells | HCV copy/cell ratio | No. of HCV RNA copies/μg |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Positive stranda |
Negative strandb |
|||||||||
| 7 dpi | 10 dpi | 14 dpi | 7 dpi | 10 dpi | 14 dpi | |||||
| 1 | 11/M | CD4+ | 5 × 106 | 1/16 | 1 × 104 | 1 × 104 | ND | ND | 5 × 103 | NT |
| CD8+ | 3.2 × 106 | 1/10 | 1 × 104 | 1 × 104 | 1 × 104 | ND | 1 × 103 | ND | ||
| 2 | 11/M | CD4+ | 3.5 × 106 | 1/10 | 3 × 102 | 2.5 × 102 | 2.5 × 102 | ND | ≤25 | ND |
| CD8+ | 3.5 × 106 | 1/10 | NA | 3 × 102 | 2.5 × 102 | NA | ≤25 | ND | ||
| 3 | 11/M | CD4+ | 5 × 106 | 1/15 | 1 × 102 | 3 × 102 | 1 × 102 | ND | ≤25 | ≤25 |
| CD8+ | 5 × 106 | 1/15 | 3 × 101 | 3 × 101 | ND | ≤25 | ND | NT | ||
| 4 | 11/M | CD4+ | 5 × 106 | 1/15 | NA | 3 × 101 | 1 × 102 | NA | ≤25 | ND |
| CD8+ | 5 × 106 | 1/15 | NA | 3 × 101 | 3 × 102 | NA | ND | 2.5 × 102 | ||
| 5 | 11/M | CD4+ | 5 × 106 | 1/15 | 1 × 103 | 3 × 101 | 3 × 101 | 2.5 × 103 | ND | ≤25 |
| CD8+ | 5 × 106 | 1/15 | NA | 1 × 104 | 1 × 104 | NA | ND | 2.5 × 102 | ||
| 6 | 16/F | CD4+ | 5 × 106 | 1/1 | NA | 1 × 104 | 1 × 104 | NA | ND | 2.5 × 103 |
| CD8+ | 5 × 106 | 1/1 | NA | 1 × 104 | 1 × 103 | NA | ≤25 | ND | ||
a
Estimated copy number of the HCV RNA positive strand detected by RT-PCR/NAH based on testing the equivalent of 1 μg total RNA.
b
Estimated copy number of the HCV RNA negative strand detected by strand-specific RT-PCR/NAH based on testing the equivalent of 1 μg of total RNA.
c
ND, not detected; NA, not available or not a sufficient amount of RNA to be tested; NT, not tested for the HCV RNA negative strand because the HCV RNA positive strand was not detected.