TABLE 3.
Parameters for detection of HCV RNA positive and negative strands in CD4+ and CD8+ T cells exposed to authentic, patient-derived HCV
Parameter | Value for HCV RNA |
|||||
---|---|---|---|---|---|---|
Positive strand |
Negative strand |
|||||
7 dpi | 10 dpi | 14 dpi | 7 dpi | 10 dpi | 14 dpi | |
CD4 cells | ||||||
No. of HCV RNA positive-strand- or negative strand-positive expts/total no. of expts completed | 4/4 | 6/6 | 5/6 | 1/4 | 4/6 | 3/5 |
Overall no. (%) of HCV RNA positive-strand- or negative-strand-positive expts/total no. of expts completed | 15/16 (93.7) | 8/15 (53.3) | ||||
Mean no. of HCV RNA copies/μg ± SEM | 2.7 × 103 ± 1.1 × 103 | 6.7 × 102 ± 3.8 × 102 | ||||
CD8 cells | ||||||
No. of HCV RNA positive-strand- or negative-strand-positive expts/total no. of expts completed | 2/2 | 6/6 | 5/6 | 1/2 | 3/6 | 2/5 |
Overall no. (%) of HCV RNA positive-strand- or negative-strand-positive expts/total no. of expts completed | 13/14 (92.8) | 6/13 (46.1) | ||||
Mean no. of HCV RNA copies/μg ± SEM | 4.4 × 103 ± 1.3 × 103 | 1.2 × 102 ± 0.8 × 102 |