FIG 3.

HPV31 E2 lysine 111 (K111) mutations repress transcription. C33A cells were cotransfected with 31-Fluc and HPV31 FLAG-tagged E2 wild type (wt) or K111 mutants. The amount of E2 at the origin was determined by ChIP using LCR4 primers (A). C33A cells were cotransfected with 31-Fluc and HPV31 FLAG-E2 wild type or K111 mutants. Repression was measured by luminometry and is shown as a fraction of baseline transcription (B). HeLa cells were transfected with HPV31 HA-E2 wild type and harvested after 48 h, and E6 transcripts were quantified by qPCR (C). *, P < 0.05.