FIG 3.
No evidence for virus infection of DC in the LN draining the site of infection in wild-type mice. (A) Viral titers in homogenates of the brachial LN measured 2 days after epidermal infection of Ifnar2−/− and WT mice with HSV-1. (B) Representative plots of MHC-IIhi CD11chi DC enriched from the brachial LN of Ifnar2−/− and WT mice infected 2 days earlier with a GFP-expressing HSV-1 strain. (C) Relative expression of viral mRNA in DC subsets isolated from the brachial LN of Ifnar2−/− and WT mice infected with HSV-1 on the skin 2 days earlier. Data are pooled results from two independent experiments (n = 5 per experiment) and are expressed as mean + SEM. N.D., not detectable.