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Estimation of the number of Env trimers per viriona
| Virus | Gag/Env ratio | Estimated no. of trimers |
|---|---|---|
| Tier 1 wt/A66-R5 | 74 | 6 |
| Tier 2 wt/A66-R5 | 55 | 8 |
| Tier 3 wt/A66-R5 | 36 | 13 |
| Tier 1 ΔCT/A66-R5 | 32 | 15 |
| Tier 2 ΔCT/A66-R5 | 33 | 14 |
| Tier 3 ΔCT/A66-R5 | 89 | 5 |
| HIV-1 BAL/SupT1-R5 | 27 | 17 |
| HIV-1 NL43/SupT1-R5 | 65 | 7 |
| SIVmac239/SupT1-R5 | 13 | 34 |
Gag p27/Env gp120 ratios were calculated, and the numbers of virion-associated trimers were approximated based on an estimate of 1,400 Gag molecules per virion.
