TABLE 2.
Overview of PB1 mutant characteristics relative to the wild typea
| Enzyme | RNP reconstitution |
Extension on vRNA | Extension on cRNA |
RNA cleavage | Trimer formation | ||
|---|---|---|---|---|---|---|---|
| vRNA | mRNA | FL | FR | ||||
| Wild type | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| S269A | + | + | +++ | +++ | +++ | NT | +++ |
| L271A | NT | NT | NT | +++ | +++ | NT | +++ |
| P272A | NT | NT | NT | − | − | NT | +++ |
| V273A | + | − | +++ | +++ | ++++ | NT | +++ |
| Δ648–651 | − | − | ++++ | ++++ | ++++ | +++ | +++ |
| Δ631–662 | − | − | − | − | − | − | + |
| Δ642–656 | − | − | ++++ | + | ++++ | +++ | +++ |
| Δ656–662 | − | − | − | − | − | − | +++ |
| Δ631–642 | − | − | + | − | +++ | + | +++ |
| Δ636–642 | − | − | +++ | ++ | ++++ | +++ | +++ |
| Δ631–635 | − | − | + | − | + | + | +++ |
a
++++, >100% of the value for the wild type; +++, 75 to 100% of the value for the wild type; ++, 50 to 75% of the value for the wild type; +, 25 to 50% of the value for the wild type; −, 0 to 25% of the value for the wild type; NT, not tested.