FIG 1.

ML effect on RV replication. (A to E) Western blot and confocal immunofluorescence analyses with the indicated antibodies of RV-infected cells (MOI, 25 VFU/cell) treated with ML at 10 μM, unless otherwise indicated, or with DMSO (D) for the indicated times. Scale bars, 10 μm. TUB, tubulin. (F) Time course of viral progeny yield of OSU-infected (MOI, 25 VFU/cell) MA104 cells treated with 10 μM ML, added at 2 hpi. The data are presented as averages ± standard deviations of the results of three independent experiments. ***, P < 0.001 (t test). (G) Genome segment analysis of blotted total RNA extracted from noninfected (NI) and OSU-infected (25 VFU/cell) MA104 cells treated with ML (10 μM) or DMSO from 1 to 8 hpi and revealed with an anti-dsRNA antibody. (H) Viability of noninfected or OSU-infected (MOI, 25 VFU/cell) MA104 cells determined by cytofluorometry of propidium iodide-stained cells following treatment at 2 hpi with or without 10 μM ML for up to 12 hpi. The data are presented as averages ± standard deviations of the results of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (t test).