TABLE 3.
Antibodies used for NK functional assay
| Antibody | Clone | Fluorochromea | Catalog no. | Company |
|---|---|---|---|---|
| Live/Dead | Near infrared | L10119 | Fisher Scientific | |
| CD45 | D058-1283 | BV786 | 563861 | BD Biosciences |
| CD3 | SP34-2 | PE CF594 | 562406 | BD Biosciences |
| CD20 | 2H7 | PE CF594 | 562295 | BD Biosciences |
| CD8 | RPA-T8 | BV711 | 563677 | BD Biosciences |
| CD16 | 3G8 | PacBlue | 558122 | BD Biosciences |
| CD56 | B169 | PerCP Cy5.5 | 560842 | BD Biosciences |
| KIR3DLO1 | NKVFS1 | PE | 130-092-688 | Miltenyi |
| NKG2A/C | Z199 | PE Cy7 | B10246 | Beckman Coulter |
| CD107a | H4A3 | BV605 | 328633 | BioLegend |
| IFN-γ | 4S.B3 | FITC | 554551 | BD Biosciences |
| TNF-α | Mab11 | Alexa Fluor 700 | 557996 | BD Biosciences |
| Granzyme B | GB11 | BV510 | 563388 | BD Biosciences |
a
PacBlue, Pacific Blue. PerCP, peridinin chlorophyll protein.