| EE cage size (cm) |
36 x54 x19 |
| EE cage composition |
polycarbonate |
| Control cage |
polycarbonate |
| EE floor space/mouse (cm 2) |
195 |
| # Mice/EE cage |
10 |
| Stimulating toys/objects in EE cage |
2 running wheels, tunnels, 2 refuges 1 swing, with nesting material |
| Objects varied regularly? |
Yes |
| Strain of mice |
C57BL/6 |
| Sex of mice |
Male |
| EE, control cages in same room? |
Yes |
| Lighting |
12 hours on/off |
| Temp (degrees C) |
22 ± 1 |
| Bedding |
Sawdust |
| Humidity control? |
No |
| Cleaning schedule |
Once a week |
| Food based on wheat, oats, meat, soy and milk? |
Yes (14% protein, 5% fat, 3041 kcal ME/kg) |
| Microbiota endemic in animal facility |
Norovirus, Helicobacter |
| Age of mice put in cage initially |
3 weeks |
| # weeks habituation |
5 weeks |
| Tumor injected |
GL261 |
| Route injected |
intrastriatal |
| # Cells injected |
7.5×104
|
| Mouse handling frequency |
Every 3 days |
| Statistical significance in tumor size? |
Yes |
