Figure 3 a,b.

a. Significantly higher gene expression of collagen 1 (Col1A1) in spheroids compared to adherent cells under microgravity (4.4x, p=0.04) and compared to the control group (5.6x, p=0.02). b. Gene expression of collagen 3 (COL3A1) was significantly increased in spheroids compared to the control group (2.3x, SD=0.37, p=0.03). c. Gene expression of the extracellular matrix gene Tenascin C (TNC) was increased in adherent cells under microgravity compared to the 1g-control group, not reaching statistical significance. d. Gene expression of the extracellular matrix gene Fibronectin (FN) was increased in adherent cells under microgravity compared to the 1g-control group, not reaching statistical significance. e. Gene expression of vimentin: no significant alteration was observed both in the adherent cells and in the spheroids compared to the 1g control group. f. Gene expression of the tenocyte-specific transcription factor scleraxis (SCX) was significantly increased in spheroids compared to the control group (3.7x, SD=0.69 p=0.03).