Detector: FAM
Quencher:None
Passive Reference: None
Run Mode: Fast 7500
Sample Volume 20 ul
| Step | Cycles | Temp | Time |
|---|---|---|---|
| Denature | 1 | 50C | 2 min |
| Hot Start | 1 | 95C | 2 min |
| PCR | 45 | 95C | 3 sec |
| 60C | 30 sec |
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