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. 2017 Dec;29(6):572–580. doi: 10.21147/j.issn.1000-9604.2017.06.12

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HOXC10 expression in gastric cancer cells. HOXC10 mRNA expression (A) and protein expression (C) were up-regulated by plasmid transfection in the AGS human gastric cancer cell line and its expression was analyzed via reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. Results are expressed as of fold changes of parental cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control; HOXC10 mRNA expression (B) and protein expression (D) at 48 h after HOXC10 silencing in the SNU638 cell line were evaluated via RT-PCR and western blotting. Results are expressed as of fold changes of parental cells. ***, P<0.001; WT, wild type; siNC, negative control.

Inline graphic — HOXC10 expression in gastric cancer cells. HOXC10 mRNA expression (A) and protein expression (C) were up-regulated by plasmid transfection in the AGS human gastric cancer cell line and its expression was analyzed via reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. Results are expressed as of fold changes of parental cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control; HOXC10 mRNA expression (B) and protein expression (D) at 48 h after HOXC10 silencing in the SNU638 cell line were evaluated via RT-PCR and western blotting. Results are expressed as of fold changes of parental cells. ***, P<0.001; WT, wild type; siNC, negative control.