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. 2017 Dec;29(6):572–580. doi: 10.21147/j.issn.1000-9604.2017.06.12

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HOXC10 effects on migration and invasion of gastric cancer cells. (A) Migration ability of HOXC10-stably transfected AGS cells was determined using a wound-healing assay. Cells were seeded in the culture inserts. After 24 h of incubation, cell-free gaps were created and images of closed gaps were taken at the indicated incubation time. The width of the wound was measured at five sites and normalized to that at 0 h; (B) HOXC10 was silenced in SNU638 cells and they were used in the wound-healing assay; (C) Invasion ability of HOXC10-stably transfected AGS cells determined by transwell invasion assay; (D) HOXC10 was silenced in SNU638 cells and they were used for the transwell invasion assay. The number of invaded cells for 24 h was counted in five representative photographs. Results are expressed as . **, P<0.01; ***, P<0.001; WT, wild type; Vector, pcDNA control; siNC, negative control.

Inline graphic — HOXC10 effects on migration and invasion of gastric cancer cells. (A) Migration ability of HOXC10-stably transfected AGS cells was determined using a wound-healing assay. Cells were seeded in the culture inserts. After 24 h of incubation, cell-free gaps were created and images of closed gaps were taken at the indicated incubation time. The width of the wound was measured at five sites and normalized to that at 0 h; (B) HOXC10 was silenced in SNU638 cells and they were used in the wound-healing assay; (C) Invasion ability of HOXC10-stably transfected AGS cells determined by transwell invasion assay; (D) HOXC10 was silenced in SNU638 cells and they were used for the transwell invasion assay. The number of invaded cells for 24 h was counted in five representative photographs. Results are expressed as . **, P<0.01; ***, P<0.001; WT, wild type; Vector, pcDNA control; siNC, negative control.