5.

Investigation of HOXC10 treatment-activated signaling molecules. (A) Protein extract (1 mg) of AGS cells with pcDNA3-HOXC10 or control plasmid was used for the phosphokinase array in accordance with the manufacturer’s instructions. The red frame is respectively labeled with No. 1 to 6, indicating different phosphorylation sites; (B, C) AGS cells were transfected with pcDNA3-HOXC10 or control plasmid. SNU638 cells were transfected with either siRNA or control. Western blotting analysis was performed after 48 h to detect p-P38, P38, p-JNK, JNK, p-ERK1/2, ERK1/2, c-Jun, P53, c-myc and RAC1 expression in AGS and SNU638 cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as internal control.