Skip to main content
. Author manuscript; available in PMC: 2019 Jan 19.
Published in final edited form as: ACS Synth Biol. 2017 Nov 20;7(1):258–266. doi: 10.1021/acssynbio.7b00293

Figure 6.

Figure 6

Analysis of WAP-8294A compounds by LC–MS. (A) The yield of WAP-8294A1, WAP-8294A2 and WAP-8294A4 in WT and engineered strains. TIC, Total Ions Chromatograph. (B) Antibacterial activity test against B. subtilis. MeOH, methanol; 1, 2, two strains of dCas9-ω3/refactored. (C) The m/z of WAP-8294A1, WAP-8294A2 and WAP-8294A4. (D) Peak area of WAP-8294A compounds in WT and engineered strains. 1, WT; 2, ΔWAPS1; 3, dCas9-ω3; 4, dCas9-ω3/refactored. Data are presented as averages of three independent experiments. *, P < 0.05; **, P < 0.01 (see Table S6).