Skip to main content
. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: J Inherit Metab Dis. 2017 Jul 19;41(1):59–72. doi: 10.1007/s10545-017-0069-8

Fig. 1.

Fig. 1

Mixed background SIRT4KO mice have decreased body weight. (A) Male (n≥12/10 wild-type/SIRT4KO at each time point) and (B) female (n≥8/12 wild-type/SIRT4KO at each time point) SIRT4KO mice were weighed at various time points over more than a year. (A, inset) Percent fat mass and lean mass were measured at 60 weeks of age (n=11/10 wild-type/SIRT4KO). Mice were fasted for 5–6 hours and then plasma samples collected for free fatty acid and glycerol measurements at various ages in male (C and E; n=13/8) and female (D and F; n=7/10) SIRT4KO mice. Plasma β-hydroxybutyrate levels were assessed in non-fasted and 24-hour fasted male (G; n=12/10) and female (H; n=9/12) SIRT4KO mice. Closed blue and red circles with a solid line indicate male wild-type and SIRT4KO mice on a mixed genetic background. Closed blue and red circles with a dashed line indicate female wild-type and SIRT4KO mice on a mixed genetic background. Data are shown as average±SEM. P values less than 0.05 by two-way ANOVA are indicated. Asterisks indicate p<0.05 between wild-type and SIRT4KO by Bonferroni’s multiple comparisons test.