Fig. 3.

Transmission electron microscopic micrographs showing the cytological effects of treatment with C. copticum extract on the ultrastructure of the midgut epithelial tissue of Cx. pipiens larvae, 24 h after treatment. (A)–(C) indicate normal nucleus (N), microvilli (MV), and chromatin contents in epithelial cells of control larvae (Scale bar = 2, 2 μm and 100 nm respectively). (D)–(F) indicate contents, degenerated microvilli (DMV) and its degenerating cell (DC) with bubbling and stretching appearance in treated larvae and represent degenerating mitochondria (DM) (Scale bar = 2, 5, and 5 μm respectively) in treated midgut cells. Ultrathin 4 μm sections were analysed with transmission electron microscope model JEOL JEM-100CX II at 80 kV.