Table 1.
PCR primers for gene expression involved in GSL and carotenoid biosynthesis in Chinese cabbage.
| Name | Sequence (5′–3′) | Amplicon (base pair) | References |
|---|---|---|---|
| MYB28_RT F | ACCATACTGTCAACACGCCTCC | 219 | Gigolashvili et al., 2007, Hirai et al., 2007 |
| MYB28_RT R | CAGAAGTGACCTTAGCCGCAAC | ||
| MYB29_RT F | CTGTCTCCTCCGTGTCTCAA | 148 | |
| MYB29_RT R | CCTCGGCTGCATTGTTACTA | ||
| MYB34_RT F | ACACCGGCGACGTCGATTC | 229 | |
| MYB34_RT R | TCTAACTCCTCCATAAGGCCAACA | ||
| MYB51_RT F | GATCTCCGAAACCAGCAAATCA | 240 | |
| MYB51_RT R | GTAGTAATGAGTGGGCCACCACT | ||
| MYB122_RT F | TTGAATGATGTTGTATCTCATGATGATG | 190 | |
| MYB122_RT R | AGTTGTCAATCCCTTCAAAGGAAACA | ||
| BrPSY_RT F | GCTATCTACGTTTGGTGCAGAAGAA | 189 | Tuan et al. (2012) |
| BrPSY_RT R | AAATGGCTGAATATCGACAGGGTAT | ||
| BrZDS_RT F | CCTTCTTGTCAAAGACCACACTCAT | 160 | |
| BrZDS_RT R | AGCTAGTGAGTTCCTCAGCTTGTCA | ||
| BrLCYB_RT F | AAGATATCCAAGAGAGGATGGTTGC | 180 | |
| BrLCYB_RT R | CCACCATGTAACCTGTAGAAGGATG | ||
| BrLCYE_RT F | ATGGATGAACAGTCTAAGCTCGTTG | 185 | |
| BrLCYE_RT R | ACACCGTAGTTGTTTGTGAAAGGAA | ||
| BrCHXB_RT F | CAGAGAAAACAAGCTCTCTGGACAC | 185 | |
| BrCHXB_RT R | CATCTGCCAAGAGAATCGGTAGTAA | ||
| BrZEP_RT F | AGACTTAAGCGCCATAAGAGGAGAA | 185 | |
| BrZEP_RT R | ACTTGACATACCAAGTGCCAGAGAC | ||
| BrNCED_RT F | CACATCCTCTGTTTTGTTCACGAC | 171 | |
| BrNCED_RT R | AAGAGTTTGTTCCTGGAGTTGTTCC | ||