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. 2018 Jan 1;16(1):27–50. doi: 10.1089/adt.2017.812

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Copyright 2018, Mary Ann Liebert, Inc.

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Fig. 8. — Effects of Cal33 cell number on fluorescent drug distribution in MCTSs: images, pseudo-color pixel intensity visualizations, and line scan fluorescent intensity profiles for (A) test drugs in MCTSs formed in ULA plates at a seeding density of 5,000 cells/well, (B) ellipticine, (C) doxorubicin, and (D) Cell Tracker Green in MCTSs formed at different seeding densities. Cal33 cells were seeded into 384-well ULA plates at seeding densities of 625, 1,250, 2,500, 5,000, 10,000, and 20,000 cells per well, and after 24 h in culture, 10 μM of test compounds was added to the MCTSs and incubated for 15 min before fixation in formaldehyde and washing with PBS. Transmitted light, FITC and TRITC images of Cal33 MCTSs were then acquired on the IXM. Transmitted light images and fluorescent images colored by the image acquisition channel, green for FITC and yellow for TRITC, are presented along with the corresponding pseudo-color pixel intensity visualizations, and line scan fluorescent intensity profiles. Representative data from one of three independent experiments are shown.

Fig. 8. — Effects of Cal33 cell number on fluorescent drug distribution in MCTSs: images, pseudo-color pixel intensity visualizations, and line scan fluorescent intensity profiles for (A) test drugs in MCTSs formed in ULA plates at a seeding density of 5,000 cells/well, (B) ellipticine, (C) doxorubicin, and (D) Cell Tracker Green in MCTSs formed at different seeding densities. Cal33 cells were seeded into 384-well ULA plates at seeding densities of 625, 1,250, 2,500, 5,000, 10,000, and 20,000 cells per well, and after 24 h in culture, 10 μM of test compounds was added to the MCTSs and incubated for 15 min before fixation in formaldehyde and washing with PBS. Transmitted light, FITC and TRITC images of Cal33 MCTSs were then acquired on the IXM. Transmitted light images and fluorescent images colored by the image acquisition channel, green for FITC and yellow for TRITC, are presented along with the corresponding pseudo-color pixel intensity visualizations, and line scan fluorescent intensity profiles. Representative data from one of three independent experiments are shown.