Fig. 9.

Effects of drug exposure times on fluorescent drug distribution in MCTSs: images, pseudo-color pixel intensity visualizations, and line scan fluorescent intensity profiles for 1 and 24 h exposure of Cal33 and FaDu MCTSs to (A) ellipticine and (B) doxorubicin. Cal33 and FaDu cells were seeded into 384-well ULA plates at 10,000 cells per well, and after 24 h in culture, 10 μM of ellipticine or doxorubicin was added to the MCTSs and incubated for either 1 or 24 h before being fixed in formaldehyde and washed with PBS. Transmitted light, FITC and TRITC images of MCTSs were then acquired on the IXM. Transmitted light images and grayscale fluorescent images are presented along with the corresponding pseudo-color pixel intensity visualizations, and line scan fluorescent intensity profiles. Representative data from one of three independent experiments are shown.