Figure 2.

IL-23 concentration and frequency of neutrophils in colonic mucosa increased after high-salt diet (HSD) consumption. C57BL/6 mice received control diet (n = 5–15) or HSD (n = 5–15) for 3 weeks. (A) IL-23 concentration in colon extract of mice fed either control diet or HSD; and (B) IFN-γ concentration in colon extract of control diet- or HSD-fed mice measured by ELISA; (C) CD4⁺ IL-23R⁺ cell frequency obtained by flow cytometry; and (D) representative dot plot of neutrophils in colons of control group (left) and HSD group (right) analyzed by flow cytometry. (E) Representative histological image of colon of control (left) or HSD fed mice (right) after 3 weeks of diet consumption (200× magnification). Graphs are representative of two independent experiments with five mice per group. Statistical analysis between groups was performed using Student’s t-test. *p < 0.05.