Figure 5.

Consumption of high-salt diet (HSD) altered expression of SGK-1. mRNA was extracted from colon of C57BL/6 mice (A) 90 min after they received a 50% NaCl solution by gavage; (B) 24 h, 36 h, 48 h, 72 h, and 120 h after consumption of either control diet (n = 5–10) or HSD (n = 5–10) and (C) after 3 weeks of control diet (n = 5–10) or HSD consumption (n = 5–10). Sgk-1 relative expression was quantified by quantitative real-time PCR in the colon extracts. Graphs are representative of three independent experiments with five mice per group. Statistical analysis between groups was performed using Student’s t-test. *p < 0.05.