FIGURE 6.

Effects of GSC on the NF-κB signaling pathway in A549 cells. (A) A549 cells were treated with GSC at different concentrations (0, 0.3, 1, 3 μM) for 24 h. Expressions of p65 in nucleus were determined by western blotting analysis. Lamin B was used as loading control. (B) The treatment of A549 cells with GSC was same as that of (A). Expressions of p65 in cytosol were determined by western blotting analysis. β-actin was used as loading control. (C) The treatment of A549 cells with GSC was same as that of (A). Expressions of p-IKK-β, IKK-β, IκBα, and p-IκBα were determined by western blotting analysis. GAPDH was used as loading control. (D) The treatment of A549 cells with GSC was same as that of (A). GAPDH was used as loading control. The results shown are representative of three different experiments.