FIGURE 7.

Gleditsia saponin C sensitizes A549 cells to TNFα-induced apoptosis by inhibiting NF-κB activity. (A) A549 cells were cultured with 50 ng/mL TNFα in the absence and presence of 1–3 μM GSC for 24 h. Protein p65 were extracted and the expression levels were determined by Western blotting analysis. Lamin B as used as loading control. Band intensities were quantified by Image J software and the results were shown representative of three different experiments. (B) A549 cells were cultured with 50 ng/mL TNFα in the absence and presence of 3 μM GSC from 0 to 120 min. Expressions of IκBα and p65 were determined by Western blotting analysis. GAPDH and Lamin B were used as loading control, respectively. (C) A549 cells were treated with GSC (3 μM), TNFα (20 ng/mL), and their combination for 24 h. Cell apoptosis ratio was determined by flow cytometry analysis. Data were representative of three independent experiments and represented as mean ± SD. ^∗p < 0.05, ^∗∗p < 0.01 (compared to no treatment group).