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. 2018 Jan 15;11:435. doi: 10.3389/fncel.2017.00435

FIGURE 6.

FIGURE 6

Adenosine inhibits N-type and P/Q-type calcium currents. (A) Effect of the L-type calcium channel blocker nifedipine (Nif, 10 μM) on calcium currents. (B) IV relationship of nifedipine-sensitive calcium currents (green graph). (C) Effect of the N-type calcium channel blocker conotoxin GVIA (CTX, 100 nM) on calcium currents. (D) IV relationship of CTX-sensitive calcium currents (yellow graph). (E) P/Q-type calcium currents were isolated by blocking N-type and L-type calcium currents with CTX + Nif. (F) IV relationship of isolated P/Q-type calcium currents (red graph). (G) Adenosine strongly reduces calcium currents in the presence of Nif. (H) In the presence of CTX and (I) CTX plus Nif, adenosine only weakly reduces calcium currents. (J) Effect of adenosine on calcium currents in the absence of calcium channel blockers (Ctrl) and in the presence of Nif, CTX and CTX plus Nif. Incubation with CTX as well as CTX plus Nif significantly reduced the adenosine-mediated attenuation on calcium currents, while Nif alone had no effect on the adenosine-mediated attenuation. n.s., not significant. ∗∗∗p < 0.001.