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. 2018 Jan 19;8:1197. doi: 10.1038/s41598-018-19680-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stability of mbp1, β-act and sdha reference genes expression, evaluated in three M. canis strains cultured on MM-Cove medium (control medium) and MM-Cove medium supplemented with keratin. Gene expression levels are represented by average C_t values. mbp1 (p = 0.91, ANOVA), β-act (p = 0.46, ANOVA) and sdha (p = 0.93, ANOVA) gene expression level was not significantly different across analyzed culture conditions. Error bars indicate standard error.