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. 2018 Jan 19;8:1205. doi: 10.1038/s41598-018-19525-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Interaction between ferredoxin I (Fd I) and the CP. (A) A yeast two-hybrid system was used to analyse the interaction between Fd I and the CP of CMV-M. Transformed Saccharomyces cerevisiae AH109 cells were cultured on SD/−Ade/−His/−Leu/−Trp/X-α-Gal medium. 1, pGADT7-RecT/pGBKT7-Lam (negative control); 2, pGADT7/pGBK-MCP; 3, pGAD-FdI/pGBKT7; 4, pGAD-FdI/pGBK-MCP; 5, pGADT7-RecT/pGBKT7-53 (positive control). (B) Bimolecular fluorescence complementation (BiFC) analysis to verify the interaction between Fd I and the CP of CMV-M in Nicotiana benthamiana epidermal cells. Two combinations of BiFC plasmids (pMCP-YFP^N/pFdI-YFP^C and pFdI-YFP^N/pMCP-YFP^C) were inserted into plant cells with Agrobacterium tumefaciens, and YFP fluorescence was detected at 3 days post-agroinfiltration. The pSPYNE-35S/pSPYCE-35S plasmids were used as controls. Scale bars = 25 µm. (C) Yeast two-hybrid system to test the interaction between Fd I and the CP of CMV-Q. 1, pGADT7-RecT/pGBKT7-Lam (negative control); 2, pGADT7/pGBK-QCP; 3, pGAD-FdI/pGBKT7; 4, pGAD-FdI/pGBK-QCP; 5, pGADT7-RecT/pGBKT7-53 (positive control). (D) BiFC to verify the interaction between Fd I and the CP of CMV-Q in N. benthamiana epidermal cells.