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. 2018 Jan 19;8:1205. doi: 10.1038/s41598-018-19525-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of Fd I levels following an infection by CMV. (A) Leaves exhibiting severe chlorosis symptoms, mild chlorosis symptoms, or vein-clearing symptoms from CMV-M–infected Nicotiana tabacum cv. Samsun plants, and asymptomatic leaves from tobacco plants infected by CMV-Q and mock-inoculated plants. (B) Western blots using anti-CP monoclonal and anti-Fd I polyclonal antibodies to analyse CMV CP and Fd I in the abovementioned samples, with actin as a control. (C) Northern blot conducted with 10 µg total RNA to examine the accumulation of Fd I transcription using a specific probe corresponding to position 75–435 of Fd I mRNA. The loading control comprised 25 S rRNA.