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. 2018 Jan 19;8:1205. doi: 10.1038/s41598-018-19525-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Symptoms induced by eliminating Fd I production. (A) Chlorosis symptoms on plants in which Fd I was silenced with a TRV-based virus-induced gene silencing system. The symptoms were similar to those induced by CMV-M on Nicotiana benthamiana plants. Additionally, there were no obvious symptoms on plants carrying the empty TRV vector. (B) Accumulation of Fd I in mock-, TRV-, and TRV-FdI–inoculated samples, as determined by western blot analysis, with plant actin as a control.