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. 2018 Jan 19;8:1205. doi: 10.1038/s41598-018-19525-5

Table 1.

Details regarding the plasmids used in this study.

Plasmids Primer Instruction
pM3QCP M-5UTR-F/M-5UTR-R, QCP-F/QCP-R, M-3UTR-F/M-3UTR-R CP mutants and based on viral infectious cDNA clones.
pQ3MCP Q-5UTR-F/M-5UTR-R, MCP-F/MCP-R, Q-3UTR-F/Q-3UTR-R
pGBK-MCP BD-MCP-F/BD-MCP-R For YTH work and based on vector pGBKT7 and pGADT7 (Clontech).
PGBK-QCP BD-QCP-F/BD-QCP-R
pGAD-FdI AD-FdI-F/AD-FdI-R
pGAD-FdI(1–55) AD-FdI-F/AD-FdI-55R
pGAD-FdI(56–145) AD-FdI-56F/AD-FdI-R
pMCP-YFPN Bi-MCP-F/Bi-MCP-R For BiFC assay and based on vector pSPYNE-35S and pSPYCE-35S36.
pMCP-YFPC
pQCP-YFPN Bi-QCP-F/Bi-QCP-R
pQCP-YFPC
pFdI-YFPN Bi-FdI-F/Bi-FdI-R
pFdI-YFPC
pFdI-GFP Sub-FdI-F/Sub-FdI-R For subcellular localization assay and based on modified vector pCAMBIA1300.
pFdI(1–55)-GFP Sub-FdI-F/Sub-FdI-55R
pTRV-FdI TRV-FdI-F/TRV-FdI-R For VIGS assay and based on TRV mediated VIGS system40.