Fig. 3.

PTRE-seq reveals the effect of the let-7 binding site on repression. a Comparison of the fold change of reporters containing synthetic let-7 binding sites with altered seed binding. Also shown are reporters containing let-7 binding sites that have perfect complement (PC) binding to let-7. Each seed binding variant is present in either one, two or four copies. The inset describes the seed binding region of each seed-binding variant site. b Table describing the natural and synthetic let-7 binding sites used in this study. MFE, minimal free energy⁷¹. mirSVR, mirSVR score⁷². c Fold change of RNA and TE for reporters containing four copies each of natural or synthetic let-7 binding sites. Boxplot whiskers indicate the furthest datum that is 1.5*Q1 (upper) or 1.5*Q3 (lower). For clarity, outliers have been removed from boxplots but were used for statistical analysis