Figure 4.

Effects of temporally controlled (ubiquitous) expression of Tßh in tßh^nM18 mutant background on flies sugar response. (A) Temperature shift 3 h before the test. Flies with a rescue construct showed an intermediate PER level, significantly different from both the mutant and the control flies, Wilcoxon rank sum test with Bonferroni correction (uncorrected p = 0.00019). (B) Temperature shifts during the starvation period, but not immediately before the test. Flies with a rescue construct behaved similarly as mutant flies. Total number of proboscis extension responses is represented in boxplots (see Figure 1). Numbers indicate sample size, asterisks denote significant difference between groups (paired Wilcoxon rank sum test, p = 0.00041 and 0.00781).