Figure 9.

Lenalidomide blocks CXC chemokine ligand 12 (CXCL12)-induced S727-STAT3 phosphorylation and IL-10 production by chronic lymphocytic leukemia (CLL) cells in vivo. PBMCs were collected from five patients treated with lenalidomide monotherapy (clinical trial 2006-0715, NCT00535873) before and after 3 months of treatment. (A) Lenalidomide therapy reduced CXCL12-induced p-S727-STAT3 in CLL cells. CLL cells were cultured in the presence or absence of 250 ng/ml of CXCL12 for 20 min. The cells were then fixed, permeabilized, and stained for p-S727-STAT3. (B) Lenalidomide therapy reduces CXCL12-induced IL-10 production by CLL cells. CLL cells were stimulated with 250 ng/ml CXC12 and cultured for 8–10 h, followed by addition of phorbol myristate acetate (PMA), ionomycin, BFA (PIB), then incubated for 6 h before performing intracellular staining.