FIGURE 1.

Amino acid sequence alignment of TaASN1, TaASN2, and TaASN3 proteins from wheat (Triticum aestivum) cv. Spark. Identical residues at the same position are highlighted in black, except for residues known to be critical for the function of the enzyme (see text), which are highlighted in red. Similar residues at the same position (conservative substitutions) are highlighted in gray. The region corresponding to peptides used to raise the two monoclonal antibodies that showed highest specificity for the asparagine synthetase proteins is indicated with a blue box.