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SDS-polyacrylamide gel electrophoresis of extracts of E. coli cells expressing wheat asparagine synthetases: TaASN1, TaASN2, and TaASN3. Expression of the proteins was induced by addition of IPTG to the bacterial cell cultures. An uninduced control is included for each protein, and each protein is also shown after purification on a nickel-nitrilotriacetic acid (Ni-NTA) agarose column. The arrow indicates the position of the expressed proteins.
