Table 3.
Epitopes used for the production of monoclonal antibodies for asparagine synthetases TaASN1, TaASN2, and TaASN3 from wheat.
| Protein | Epitope identified from GenBank entries | Corresponding sequence in TaASN1–3 from cv. Spark | Position in protein |
|---|---|---|---|
| TaASN1 (GenBank AY621539) | HLPATIMAGTSK | HLPATILTGTSK | 558–569 |
| IMAGTSKKPRMI | ILTGTSKKPRMI | 563–574 | |
| SKKPRMIEVAAP | SKKPRMIEVAAP | 568–579 | |
| MIEVAAPGVAIES | MIEVAAPGVAIES | 573–585 | |
| TaASN2 (GenBank BT009049) | TVAVGGSNKPGV | TVAVGGSNKPGV | 558–569 |
| GGSNKPGVMNTV | GGSNKPGVMNTV | 562–573 | |
| KPGVMNTVVPGV | KPGVMNTVVPGV | 566–577 | |
| MNTVVPGVAIET | MNTVVPGVAIET | 570–581 | |
| TaASN3 (GenBank AK333183) | KAPASADPVFRP | KAPASVDPVLENAFHP | 558–573 |
| DPVFRPPAHGES | DPVLENAFHPPAHGES | 564–579 | |
| PAHGESILVETG | PAHGESTLVKSA | 574–585 | |
| ILVETGVPAAAV | TLVKSAVPAAAV | 580–591 |
Underlined residues show where the sequences from cv. Spark differ from those in the GenBank entries.