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. 2018 Jan 15;8:2700. doi: 10.3389/fmicb.2017.02700

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Copyright © 2018 Niaz, Shabbir, Noor, Abbasi, Raza and Imran.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytotoxicity analysis of nano formulations. (A) In vitro hemolysis assay on human blood cells. Evaluation of different formulation e.g., nisin, nisin loaded NLs and nisin loaded MCCS for relative % hemolysis, (B) Cytotoxicity analysis on HepG2 liver cells treated with (I) untreated HepG2 cells (II) nisin (III) nisin loaded liposomes (IV) nisin loaded multicomponent colloidal system, (C) Relative % cell viability of different nano-formulations against HepG2 determined by using SRB; following treatment with free nisin, nisin loaded liposomes and nisin loaded MCCS. All assay values were taken in triplicate and mean values ± SD were calculated.