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. 2018 Jan 19;8:1242. doi: 10.1038/s41598-018-19668-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The arrow indicates the site of the optodes for the NIRS procedure on an MRI T2 weighted image (a, top). The circular regions of interest were placed manually at the site presumed to be measured by NIRS, and rCBF and rOEF were measured on the result of a ¹⁵O-labeled gas PET study (a, bottom). A representative ICG time-intensity curve showing the parameters used to estimate the regional cerebral perfusion. We set the zero point as the time to rise to +4 standard deviation of the average ICG concentration in the period 0–10 seconds after intravenous injection, and calculated ΔICGmax (the maximum ICG concentration), TTP (time to peak: the time between 0 and 100% of the maximum ICG concentration), RT (rise time: the time between 10 and 90% of the maximum ICG concentration), and BFI(blood flow index: ΔICGmax / RT). Each measured parameter was normalized using the affected-to-unaffected side ratio (b).