Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 15;8:233. doi: 10.3389/fgene.2017.00233

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Prince and Rand.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

E(spl)mδ overexpression in myogenic lineage perturbs IFM development. (A) Expression levels of E(spl)mδ using two independent constructs, UAS-mδ h8 and UAS-mδ ORF, was assessed by RT-qPCR with RNA from p5 pupae (^**p ≤ 0.01, ^***p < 0.001 in comparison to Mef2-G4; #p ≤ 0.05, in comparison to Mef2 > mδ h8, one-way ANOVA). (B) Eclosion of Mef2 > mδ h8 and Mef2 > mδ ORF (^***p < 0.001 in comparison to Mef2-G4; ###p < 0.001 in comparison to Mef2 > mδ h8, z-test). (C–N) Developing IFMs in pupae at indicated stages were imaged by epifluorescence. Overexpression of E(spl)mδ was compared to knockdown of the myoblast fusion protein Sticks and Stones (SNS). (C–E) Mef2-RFP (control), (F–H) Mef2-RFP > mδ ORF, (I–K) Mef2-RFP > mδ h8, and (L–N) Mef2-RFP > SNS RNAi pupae were dissected from their pupal cases and imaged directly. Open arrows point to IFMs undergoing extension. White arrows point to partially formed DLM fibers. Asterisk mark absence of DVM formation.