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. 2018 Jan 19;8:1189. doi: 10.1038/s41598-017-19114-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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siPOU5F1 facilitates the upregulation of relevant tissue-specific markers mediated by other transcription factor synRNAs. (a) Experimental schematic for (b). hESC/iPSCs were transfected with siCtrl or siPOU5F1 and synRNAs for HNF1A, RUNX2, and SOX9 at the indicated time points. Emerald synRNA was used as a control. A mixture of siRNA and synRNA was transfected on the first day. On day 2, only synRNA was transfected twice. The cells were cultured in ES maintenance medium and sampled for qRT-PCR analysis on day 2. (b) Relative gene expression levels of tissue-specific genes (AFP, COL1A1, and COL2A1) in hESCs (SEES3) and hiPSCs (409B2) transfected with siCtrl or siPOU5F1 and synRNA. The transfected synRNA of transcription factors are indicated on the horizontal axis. Em indicates Emerald synRNA. The expression levels were normalized against that of GAPDH. Error bars indicate SEM (n = 3). *P < 0.05, t-test.