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. 2018 Jan 19;8:1264. doi: 10.1038/s41598-018-19704-4

Figure 1.

Figure 1

miR-155 expression was induced by dexamethasone, but not rosiglitazone, in differentiating preadipocytes. (a) Murine 3T3-L1 preadipocytes were induced to differentiate in the presence of 500 µM IBMX (M), 100 nM insulin (I) and supplemented with either solvent control (MI) or 250 nM dexamethasone (MID). (b) Murine 3T3-L1 preadipocytes were induced to differentiate in the presence of 500 µM IBMX (M), 100 nM insulin (I) and supplemented with either solvent control (MI) or 5 μM rosiglitazone (MIR). (c) Murine 3T3-L1 preadipocytes were treated with 500 µM IBMX (M) and/or 100 nM insulin (I). Otherwise, preadipocytes were left in media, not including differentiation inducers (preadipocytes). After 24 hours, miRNA was isolated, and the expression levels of miR-155 were quantified by real-time qPCR. Levels were normalized to endogenous RNU6 and expressed as fold over MI. Results from 3 separate experiments are graphically represented as mean ± S.E.M. *Denotes p < 0.05, **denotes p < 0.01, and ***denotes p < 0.001, as assessed by paired t-test (a and b) or one-way ANOVA with Tukey’s post-hoc tests (c).